{"id":108,"date":"2018-11-30T14:24:45","date_gmt":"2018-11-30T14:24:45","guid":{"rendered":"https:\/\/admin.innaxon.co.uk\/?page_id=108"},"modified":"2018-11-30T14:28:29","modified_gmt":"2018-11-30T14:28:29","slug":"monophosphoryl-lipid-a-mpla-bio-synthetic-from-s-minnesota-r595-re-tlrpure-sterile-solution","status":"publish","type":"page","link":"https:\/\/wp.innaxon.co.uk\/?page_id=108","title":{"rendered":"Monophosphoryl Lipid A [MPLA] (bio-synthetic) from S. minnesota R595 (Re) TLRpure Sterile Solution"},"content":{"rendered":"

Product Size<\/strong><\/p>\n

250\u00b5g:\u00a0\u00a3100.91<\/span><\/p>\n

500\u00b5g: \u00a3134.55<\/span><\/p>\n

1mg: \u00a3201.82<\/span><\/p>\n

 <\/p>\n

Product Number<\/strong><\/p>\n

250\u00b5g: IAX-100-002-C250<\/p>\n

500\u00b5g: IAX-100-002-C500<\/p>\n

1mg: IAX-100-002-M001<\/p>\n

 <\/p>\n

Source <\/b>
\nMonophosphoryl Lipid A [MPLA] derived from S. minnesota <\/i>R595 (Re) TLRpure\u2122 LPS (bio-synthetic).<\/p>\n

TLRpure\u2122 <\/b>
\nNo detectable TLR4 independent <\/i>activity: standardised potent TLR4-specific agonist.<\/p>\n

Purity <\/b>
\n\u226599.9%. No detectable DNA, RNA and protein traces.<\/p>\n

Purification Method <\/b>
\nR-form (mutant\/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure\u2122 LPS, from which MPLA was generated by acid hydrolysis.<\/p>\n

Concentration\u00a0\u00a0 <\/b>
\n1mg\/ml (0.5mg\/ml for 250\u03bcg size) stabilised in sterile, double-distilled water (ddWater), without any additives.<\/p>\n

Biological Activity <\/b>
\nOptimal concentration is dependent upon cell type, species, desired activation and analysis: 0.1-1.0\u03bcg\/ml. Does not activate any TLR other than TLR4 as tested up to 50\u03bcg\/ml in relevant cellular systems (macrophages).<\/p>\n

Shipping\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/b>
\nAMBIENT<\/p>\n

Storage <\/b>
\n+4\u00b0C<\/p>\n

Handling Advice <\/b>
\nDo not freeze. Prepare diluted MPLA working solutions in water just prior to use, keep sterile. Do not <\/i><\/b>pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of MPLA. To yield a 100\u03bcg\/ml (1,000-100x) stock solution add 100\u03bcl of MPLA to 900\u03bcl endotoxin-free sterile\u00a0 ddWater (Cat. No.: IAX-900-002) (not <\/i><\/b>PBS) and mix well. Ready-made solution is cell culture-grade.<\/p>\n

Stability <\/b>
\nStable for at least 2 years after receipt when stored at +4\u00b0C.<\/p>\n

 <\/p>\n

We ensure that our products fulfill the following:<\/b><\/p>\n