{"id":91,"date":"2018-11-30T13:35:06","date_gmt":"2018-11-30T13:35:06","guid":{"rendered":"https:\/\/admin.innaxon.co.uk\/?page_id=91"},"modified":"2018-11-30T13:35:06","modified_gmt":"2018-11-30T13:35:06","slug":"lipid-a-from-e-coli-r515-re-tlrpure-sterile-solution","status":"publish","type":"page","link":"https:\/\/wp.innaxon.co.uk\/?page_id=91","title":{"rendered":"Lipid A from E. coli R515 (Re) TLRpure Sterile Solution"},"content":{"rendered":"

Product Sizes<\/strong><\/p>\n

250\u00b5:\u00a0\u00a372.89 (VAT exempt)<\/span><\/p>\n

500\u00b5:\u00a0\u00a3100.91\u00a0(VAT exempt)<\/span><\/p>\n

1 mg:\u00a0\u00a3156.98 (VAT exempt)<\/span><\/p>\n

 <\/p>\n

Product Number<\/strong><\/p>\n

250\u00b5: IAX-100-004-C250<\/p>\n

500\u00b5: IAX-100-004-C500<\/p>\n

1 mg: IAX-100-004-M001<\/p>\n

 <\/p>\n

Source <\/b>
\nLipid A (hexa-acyl) from E. coli <\/i>R515 (Re) TLRpure\u2122 LPS (biosynthetic).<\/p>\n

TLRpure\u2122 <\/b>
\nNo detectable TLR4 independent <\/i>activity: standardised potent TLR4-specific agonist.<\/p>\n

Purity <\/b>
\n\u226599.9%. No detectable DNA, RNA and protein traces.<\/p>\n

Purification Method <\/b>
\nR-form (mutant\/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure\u2122 LPS, from which Lipid A (hexa-acylated) was generated by acid hydrolysis.<\/p>\n

Concentration\u00a0\u00a0 <\/b>
\n1mg\/ml (0.5mg\/ml for 250\u03bcg size) stabilised in sterile, double-distilled water (ddWater), without any additives.<\/p>\n

Biological Activity <\/b>
\nOptimal concentration is dependent upon cell type, species, desired activation and analysis: 0.1-1.0\u03bcg\/ml. Does not activate any TLR other than TLR4 as tested up to 50\u03bcg\/ml in relevant cellular systems (macrophages).<\/p>\n

Shipping\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/b>
\nAMBIENT<\/p>\n

Storage <\/b>
\n+4\u00b0C<\/p>\n

Handling Advice <\/b>
\nDo not freeze. Prepare diluted Lipid A working solutions in water just prior to use, keep sterile. Do not <\/i>pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of Lipid A.\u00a0To yield a 100\u03bcg\/ml (1,000-100x) stock solution add 100\u03bcl of Lipid A to 900\u03bcl endotoxin-free sterile\u00a0ddWater (Cat. No.: IAX-900-002) (not<\/i> <\/i><\/b>PBS) and mix well. Ready-made solution is cell culture-grade.<\/p>\n

Stability <\/b>
\nStable for at least 2 years after receipt when stored at +4\u00b0C.<\/p>\n

 <\/p>\n

We ensure that our products fulfill the following:<\/b><\/p>\n