£251.39

 

Explorer™ Set contains

  • Lipopolysaccharide (LPS) from E. coli O8:K27 S-form TLRpure™ Sterile Solution, 500μg (Cat. No.: IAX-100-006-C500).
  • Lipopolysaccharide (LPS) from E. coli R515 (Re) TLRpure™ Sterile Solution, 500μg (Cat. No.: IAX-100-007-C500).
  • Monophosphoryl Lipid A [MPLA] from E. coli R515 (Re) TLRpure™ Sterile Solution, 250μg (Cat. No.: IAX-100-003-C250).

TLRpure™
No detectable TLR4 independent activity: standardised potent TLR4-specific agonist.

Purity
≥99.9%. No detectable DNA, RNA and protein traces.

Purification Method

S-form LPS S-form LPS was isolated by the hot phenol-water method followed by a modified phenol-chloroform-petroleumether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS.

R-form LPS R-form (mutant/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS.

MPLA MPLA was generated by acid hydrolysis from R-form TLRpure™ LPS.

Concentration  
1mg/ml (0.5mg/ml for 250μg size) stabilised in sterile, double-distilled water (ddWater), without any additives.

Biological Activity
Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.01-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).

Shipping            
AMBIENT

Storage
+4°C

Handling Advice
Do not freeze. Prepare diluted MPLA working solutions in water just prior to use, keep sterile. Do not pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of MPLA. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of Lipid A to 900μl endotoxin-free sterile ddWater (Cat. No.: IAX-900-002) (not PBS) and mix well. Ready-made solution is cell culture-grade.

Stability
Stable for at least 2 years after receipt when stored at +4°C.

 

We ensure that our products fulfill the following:

  • Qualified Purity & Activity
  • High potency TLR4-specific Ligand
  • Ultrapure (no detectable protein, RNA and DNA)
  • Tested on TLR4 KO murine macrophages
  • Standardised Aqueous Sterile Solution
  • No purification or hazardous solubilisation
  • Excellent lot-to-lot consistency