£361.09

 

Explorer™ Set I contains

  • Lipopolysaccharide (LPS) from S. minnesota S-form) TLRpure™ Sterile Solution, 500μg (Cat. No.: IAX-100-020-C500).
  • Lipopolysaccharide (LPS) from S. minnesota R60 (Ra) TLRpure™ Sterile Solution, 500μg (Cat. No.: IAX-100-016-C500).
  • Lipopolysaccharide (LPS) from S. minnesota R595 (Re) TLRpure™ Sterile Solution, 500μg (Cat. No.: IAX-100-008-C500).
  • Lipid A from S. minnesota R595 (Re) TLRpure™ Sterile Solution, 250μg (Cat. No.: IAX-100-001-C250).
  • Monophosphoryl Lipid A [MPLA] from S. minnesota R595 (Re) TLRpureTM Sterile Solution, 250μg (Cat. No.: IAX-100-002-C250).

TLRpure™
No detectable TLR4 independent activity: standardised potent TLR4-specific agonist.

Purity
≥99.9%. No detectable DNA, RNA and protein traces.

Purification Method

S-form LPS S-form LPS was isolated by the hot phenol-water method followed by a modified phenol-chloroform-petroleumether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS.

R-form LPS R-form (mutant/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS.

MPLA MPLA was generated by acid hydrolysis from R-form TLRpure™ LPS.

Lipid A MPLA was generated by mild acid hydrolysis from R-form TLRpure™ LPS.

Concentration  
1mg/ml (0.5mg/ml for 250μg size) stabilised in sterile, double-distilled water (ddWater), (Cat. No.: IAX-900-002), without any additives.

Biological Activity
Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.01-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).

Shipping            
AMBIENT

Storage
+4°C

Handling Advice
Do not freeze. Prepare diluted MPLA/Lipid A working solutions in water just prior to use, keep sterile. Do not pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of MPLA/Lipid A.

Stability
Stable for at least 2 years after receipt when stored at +4°C.

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We ensure that our products fulfill the following:

  • Qualified Purity & Activity
  • High potency TLR4-specific Ligand
  • Ultrapure (no detectable protein, RNA and DNA)
  • Tested on TLR4 KO murine macrophages
  • Standardised Aqueous Sterile Solution
  • No purification or hazardous solubilisation
  • Excellent lot-to-lot consistency