Product Sizes
250µ: £72.89 (VAT exempt)
500µ: £100.91 (VAT exempt)
1 mg: £156.98 (VAT exempt)
Product Number
250µ: IAX-100-004-C250
500µ: IAX-100-004-C500
1 mg: IAX-100-004-M001
Source
Lipid A (hexa-acyl) from E. coli R515 (Re) TLRpure™ LPS (biosynthetic).
TLRpure™
No detectable TLR4 independent activity: standardised potent TLR4-specific agonist.
Purity
≥99.9%. No detectable DNA, RNA and protein traces.
Purification Method
R-form (mutant/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS, from which Lipid A (hexa-acylated) was generated by acid hydrolysis.
Concentration
1mg/ml (0.5mg/ml for 250μg size) stabilised in sterile, double-distilled water (ddWater), without any additives.
Biological Activity
Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.1-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).
Shipping
AMBIENT
Storage
+4°C
Handling Advice
Do not freeze. Prepare diluted Lipid A working solutions in water just prior to use, keep sterile. Do not pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of Lipid A. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of Lipid A to 900μl endotoxin-free sterile ddWater (Cat. No.: IAX-900-002) (not PBS) and mix well. Ready-made solution is cell culture-grade.
Stability
Stable for at least 2 years after receipt when stored at +4°C.
We ensure that our products fulfill the following:
- Qualified Purity & Activity
- High potency TLR4-specific Ligand
- Ultrapure (no detectable protein, RNA and DNA)
- Tested on TLR4 KO murine macrophages
- Standardised Aqueous Sterile Solution
- No purification or hazardous solubilisation
- Excellent lot-to-lot consistency