Product Size

250µg: £72.89

500µg: £100.91

1mg: £156.98

 

Product Number

250µg: IAX-100-001-C250

500µg: IAX-100-001-C500

1mg: IAX-100-001-M001

 

Source
Lipid A (hepta-acyl) derived from S. minnesota R595 (Re) TLRpure^TM LPS (biosynthetic).

TLRpure™
No detectable TLR4 independent activity: standardised potent TLR4-specific agonist.

Purity
≥99.9%. No detectable DNA, RNA and protein traces.

Purification Method
R-form (mutant/rough) LPS was isolated by phenol-chloroform-petroleum-ether method. Semi-purified LPS was subjected to further re-extraction cycles and ultracentrifugation steps, extensively electrodialysed before converted to its uniform sodium salt form to yield TLRpure™ LPS, from which Lipid A was generated by acid hydrolysis.

Concentration  
1mg/ml (0.5mg/ml for 250μg size) stabilised in sterile, double-distilled water (ddWater), without any additives.

Biological Activity
Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.1-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).

Shipping            
AMBIENT

Storage
+4°C

Handling Advice
Do not freeze. Prepare diluted Lipid A working solutions in water just prior to use, keep sterile. Do not pre-dilute in buffer (e.g. PBS) as this will lead to precipitation of Lipid A. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of Lipid A to 900μl endotoxin-free sterile ddWater (Cat. No.: IAX-900-002) (not PBS) and mix well. Ready-made solution is cell culture-grade.

Stability
Stable for at least 2 years after receipt when stored at +4°C.

 

We ensure that our products fulfill the following:

• Qualified Purity & Activity
• High potency TLR4-specific Ligand
• Ultrapure (no detectable protein, RNA and DNA)
• Tested on TLR4 KO murine macrophages
• Standardised Aqueous Sterile Solution
• No purification or hazardous solubilisation
• Excellent lot-to-lot consistency